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Phis2-p53

Webb4 okt. 2013 · The interactions of pHIS2-p53 (three tandem copies of the cis-acting DNA consensus sequence in pHIS2, which is recognized by p53) with the tested TFs were used as negative controls. Two MYC ( ThMYC4 and ThMYC6 GenBank number: JN166788 and JN166790) were identified to bind to the E-box motif, of which ThMYC6 that bound with … Webb24 jan. 2024 · The p53 fragment was from pGBKT7-p53 used for Y2H. ... The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT.

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

Webbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 … Webb3 mars 2024 · The pHIS2-p53/pGADT7-p53 and pHIS2-p53/pGADT7-PsnSHN2 were used as the positive and negative control, respectively. 1, 10 −2 and 10 −4 represent solution dilution ratio of transformed Y187 ... nothelferkurs ruswil https://dcmarketplace.net

Subcellular localization and binding elements analyses. (a) …

Webb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … WebbPGADT7-p53 binding to pHIS2-p53 (AD-Rec2-p53+ pHIS2-p53) used as the positive control, AD-Rec2-ScAPD1-like and pHIS2-p53 were used as the negative control. Source publication WebbEngineered Process nothelferkurs san arena

pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …

Category:P0153: Code Meaning, Causes, Symptoms, & Tech Notes - Engine …

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Phis2-p53

p73 or p53 Directly Regulates Human p53 Transcription to …

http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf Webb酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，. 产品型号：. 产品时间： 2015-11-08. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯，pQE 载体，原核表达载体，克隆载体 ，毕赤酵母表达载体，毕赤 ...

Phis2-p53

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Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 … Webbp53his载体价格1200元，p53his载体质粒图谱(Vector map),载体序列(Sequence)见下文。p53his是酵母单杂交载体的p53his。

Webb1）准备质粒：构建pHis2-bait质粒→提取质粒； 2）制备Y187感受态细胞：YPDA平板活化→YPDA液体扩繁→制备酵母感受态细胞；或者直接购买Y187感受态细胞（CC306）； … Webb27 apr. 2024 · The glycogen synthase 2 (Gys2) and p53 generates a novel negative feedback circuit in which p53 represses Gys2 expression whereas Gys2 can stabilize …

http://www.labbase.net/Supply/SupplyItems-4229347.html Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by …

Webb上海北诺生物科技有限公司所提供的酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，质量可靠、规格齐全,上海北诺生物科技有限公司不仅具有专业的技术水平,更有良好的售后服务和优 …

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html how to set up an expiration date in excelWebbpHIS2-p53+pGADT7-53m（阳性对照） pHIS2+pGADT7-53m（阴性对照） 服务特色：经验足 周期短 性价比高 通量高. 客户提供. 构建酵母文库材料. 细胞样本：细胞数量＞ … how to set up an external keyboardWebbOur Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbA r) as a stringent, highly selective, and easy-to-use reporter. nothelferkurs solothurn noheWebb1 mars 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PtrHAT22 were used as the positive and negative control, respectively. 10 −1, 10 −2, and 10 −3 represent solution dilution ratio of transformed Y187 yeast cells. 4. Discussion. how to set up an ez up canopyWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … nothelferkurs surseeWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … how to set up an external displayhttp://www.bnbiotech.com/beinuobio-Products-11104093/ nothelferkurs sg