WebDec 14, 2016 · Uracil-DNA glycosylase (UNG) is a DNA repair enzyme that can recognize and remove uracil residues from DNA . In 1990, the use of UNG to inactivate PCR products … WebThe enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity. Longo, M.C. et al. (1990) Gene 93, 125–8.
AmpErase™ Uracil N-Glycosylase (UNG) - Thermo Fisher Scientific
WebJan 1, 1997 · the use of the dUTP/UNG carryover prevention strategy. Substitution of dUTP for dTTP in a R T/PCR amplification also containing dITP did not result in loss of sensitivity compared with WebDec 1, 1996 · However, when using the dUTP/UNG carryover prevention system, the Tann must be maintained above 50°C, since high temperature denaturations are not utilized and UNG is active at or below temperatures of 50°C and will degrade DNA containing dUMP. The use of mixtures of dITP and dGTP allows for modulation of the Tann. csc mc 14 s. 1991
What is UNG/UDG? Thermo Fisher Scientific - HK
WebBesides, carry-over contamination is a serious problem in diagnostic PCR assays. To improve the run time and reliability of the endpoint PCR test, we developed a two-step … As PCR can amplify such tiny amounts of DNA, preventing contamination is essential: even small amounts of contamination can produce false positives in your experiments. Contamination can include cross-contamination from other samples, DNA contamination from elsewhere in the laboratory, and … See more UNG is active on single- and double-stranded dU-containing DNA, but dUTP is not a substrate for UNG. Taq polymerase and other components of the PCR mixture are … See more Despite the advantages that UNG offers, E. coli UNG is not fully heat-deactivated and can degrade PCR products over time, which will affect the results of your … See more 1. Delort AM, Duplaa AM, Molko D et al. (1985) Excision of uracil residues in DNA: Mechanism of action of Escherichia coli and Micrococcus luteus uracil … See more WebIncorporate this glycosylase in your PCR containing dUTPs to minimize contamination from carryover products. We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. ... UNG Plus; TaKaRa PCR Carryover Prevention Kit; Uracil DNA Glycosylase (UNG), Heat-Labile ... dyson animal new model