Cifwt
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Cifwt
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Web(B) Anti-biotin western blot showing titration of the indicated amount of Cifwt or CifC/A … Web(B) Anti-biotin western blot showing titration of the indicated amount of Cifwt or CifC/A with 200 nM SCFFbw7ΔD, prior to 10-min long polyubiquitylation reactions for a biotin-labeled cyclin E phosphopeptide. In all these reactions, the indicated version of Cif was preincubated with NEDD8-modified SCFFbw7ΔD for 1 h prior to reaction initiation.
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