WebAug 11, 2024 · After examining the amplified PCR products by 1.5% agarose gel with EcoDye Nucleic acid staining solution (BioFact, Daejeon, Korea), it was purified by PCR purification kit (Favorgen, Pingtung country, Taiwan). The purified PCR products were sequenced in the bidirection using ABI3500 Genetic analyzer (Thermo Fisher Scientific, … WebBioFact™ 2X Real-Time PCR Master Mix (25mM MgCl 2), with EvaGreen™ 5 ㎖ 1 ㎖ x 4 ea. DQ381-40h. BioFact™ 2X Real-Time PCR Smart mix (final 2.5mM MgCl 2), including Evagreeen™ 5 ㎖ in mixture. 1 ㎖ x 4 ea. DQ722-25h. BioFact™ 2X Multiplex Real-Time PCR Smart mix for Probe. 0.5 ㎖ x 5 ea (250 reactions) 31000-500. 20X EvaGreen ...
Biofact (biology) - Wikipedia
WebSep 10, 2024 · The samples were then separated using 1% agarose gels, and stained with Ecodye (Biofact, Korea) to visualize migration shifts. To significantly improve the separtion resolution of DNA fragments the percentage of agarose was increased to 2%. 3. Results and discussion3.1. Molecular characterization of the Vip3Aa protein. sibme learning center
Investigation of Hanwoo-specific structural variations using whole ...
Webphoresis on 2% agarose gels containing EcoDye DNA Staining Solution (Biofact, Korea). Chromosome counting Chromosome analyses of embryos were performed using general procedures described previously, with modifications [13]. Embryos were collected and cultured for 6 h in PZM-3 medium contain-Table 1. Primers utilized for gene expression … WebSep 7, 2024 · EcoDye™ Nucleic Acid Staining Solution 201 Membrane filter BioFACT™ 1kb Plus DNA Ladder 204 MCE (Mixed Cellulose Ester) 237 BioFACT™ 100 bp Plus DNA … WebNov 25, 2013 · EcoDye™ DNA Staining Solution is a stable and sensitive DNA fluorescent dye. It is non-mutagenic and non-cytotoxic and can be used instead of Ethidium Bromide … sibm disease latest news 2023